The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself the 7th country in world with number of people occupies rank 7 Apart from that, robusta coffee (Coffea canephora L) one most popular drinks globally, including Indonesia. Chlorogenic acid beans effectively reduces cell due to free radicals, minimizing excessive release liver into blood. Objective: This study aimed analyze effects chlorogenic ethanolic fraction on Wistar rats. Method: researchers applied an experimental a randomized pretest-posttest control group design. were extracted using Maceration method then fractionated hexane ethyl acetate solvent. concentration obtained remaining was measured spectrophotometer. Furthermore, hyperglycemia testing included 30 rats induced 20% for 3 – 4 weeks. They given ethanol dose 400 mg/kg BW 500 BW. Meanwhile, metformin served positive control, NaCMC negative control. Results: analysis spectrophotometer 37% indicated decrease 16.66% 48.06% each 51.53% 52.16%, respectively. Conclusion: significantly affects